ABI PRISM AUTOMETIC GENETIC ANALYZER MODEL 310-3 자동 염기서열 분석기
상태양호
연락처 010-5354-6248

ABI PRISM？
310기기는 single-capillary로 sequencing 실험과 fragment analysis 실험 모두를 지원하는 Automated Genetic Analyzer입니다. 이 기기를 이용하면 de novo sequencing 실험에서부터SNP validation linkage mapping 실험까지 다양한 application의 실험을 할 수 있습니다.

1. Capillary electrophoresis 방식을 이용한 세계최초의 DNA analyzer
2. 72시간 unattended operation
3. Automated systems으로 시간 및 labor costs를 줄임
4. Throughput (maximum):
(1) Sequencing throughput– 9120 basecalls (24 runs x 1 capillary x 380 basecalls per run)
(2) DNA size throughput- 720 genotyping analyses (48 runs x 1 capillary x 15 genotyping analyses per run)
5. Application: Comparative sequencing linkage analysis SNP discovery and validation mutation detection등

Standard-Mode Performance: 98.5% basecalling accuracy
Standard-Mode Throughput: 5220 basecalls (9 runs x 580 basecalls per run)
Rapid-Mode Performance: 98.5% basecalling accuracy
Rapid-Mode Throughput: 9120 basecalls (24 runs x 380 basecalls per run)
DNAsize Performance: Single base detection up to 250 bases with 0.15 Standard deviation; 2 base detection between 250-350 bases with 0.3 Standard deviation
DNAsize Throughput: 720 genotyping analyses (48 runs x 1 capillary x 15 genotyping analyses per run)
MUTATIONVS Performance: 0.3% CV pattern-matching precision or better
MUTATIONVS Throughput: 72 single-stranded confirmation polymorphism (SSCP) samples (forward and reverse strand differentially labeled)

메뉴얼
http://www.units.muohio.edu/cbfg/Files/ABI310userman.pdf