BISCHOFF LAMBDA 1010 PRINCE TECHNOLOGIES CE SYSTEM 판매합니다
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The PrinCE-C 650 and 660 are the top of flexibility in Capillary Electrophoresis. Both these systems are added with a second buffer lift. This second lift is used to allow the change of outlet buffers at will.

Because of the dual lift approach and the possibilities for external pressure up to 10bars the PrinCE-C 650 and 660 are the most versatile of all PrinCE instruments. It is ideally suited for method development and routine analysis by one or more methods after another. The latter can be extremely useful if not all analytes in a sample can be separated with a single buffer. The PrinCE-C 660 offers the possibility of sample and buffer cooling.

Both PrinCE systems are fully automated and can handle up to 32 (or optional 40) samples without interference of the operator while still maintaining 10 (or optional 5) buffer vials. Any combination of six segments (sample/buffer type) can be used. The operator can exchange the segments easily without needing any tools.

Under precisely controlled circumstances it is possible to use the PrinCE-C 650 and 660 for fraction collection. The detector will typically be placed on top of the Prince which will reduce bench space. The extra features of PrinCE-C 650 compared to PrinCE-C 660 is the samples and buffers cooling.

Main specifications:

- Flushing pressure
0 to +2500mbar by DCI up to 10 bars by external pressure


- Voltage range
-30kV to +30kV 100V increments

- Current range
-200μA to +200μA 1μA increments


- Ramping
Programmable voltage or current ramping


- Polarity reversal
Programmable pre-run and during the run


- Autosampler
30/48-position carousel sample and buffer vials. Variable vial sizes for samples and buffers available including Eppendorf micro centrifuge tubes


- Temperature control
Capillary 5°C below ambient to 60°C (PrinCE-C 650 and 660)
Samples and buffers: 4°C to 40°C (PrinCE-C 660)


- Detector
Allows the use of any CE-detector including MS Fluorescence LIF Conductivity UV/VIS and Photo Diode Array


- Software
Allows the use of any data station. Optional: Our DAx Data Acquisition and Analysis software. This software is specially made for our PrinCE systems




Injection modes Hydrodynamic injection uses patended Dynamic Compression Injection (DCI) to generate and apply a range of positive and negative pressures with controlled ramping (-180 to 250mbar 1mbar resolution). Electrokinetic Injection features controlled ramping in voltage or current mode (max. 30 kV). Repeatability of injected volume: < 1.0%RSD with n > 10.


Ordering information rhkdvk Flyer rhkdvk Brochure






Principle of Operation - UV-VIS Detection

UV absorbance detection is the most popular form of detection. More than 50 % of all CE systems are equipped with UV absorbance detectors. Among the advantages of UV-VIS detectors are simplicity of use and the fact that almost any compound can be detected by UV absorbance either direct or indirect. Typical limits of detection are 1 to 10 μM for compounds that show considerable absorbance at least one wavelength. Out of many UV-VIS detectors that are used in combination with PrinCE Prince Technologies offers one of the most sensitive detectors the Lambda 1010 UV-VIS Detector.


Some Features of the Lambda 1010 UV-VIS Detector


Monochromator based wavelength selection in steps of 1 nm
Simple operation conditions and compact design
High Sensitive Special designed CE-cell
Wavelength Switching
Detection at the absorbance maximum
On column measurement


Main Specifications:

- Wavelength
190-800nm

- Wavelength accuracy
± 2 nm

- Wavelength reproducibility
± 0.2 nm

- Optical bandwidth
6 nm

- Noise at 230 nm cell filled with water
± 10-5 AU

- Measuring range incremental by 0.001
0.001 - 1.999 AU




Monochromator based wavelength selection
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Many UV/VIS detectors are equipped with filters for wavelength selection. This limits the selection of wavelengths which may cause measuring next to the maximum of absorption. The Lambda 1010 detector uses a monochromator for wavelength selection making it possible to choose from each wavelength in the range of 190 - 800 nm.


Simple operation conditions and compact design
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The Lambda 1010 is a programmable UV/VIS detector for CE. The advanced technology and the user-friendly design of the instrument generate superb results in routine and more sophisticated chromatographic applications.


High Sensitive special designed CE Cell by Prince Technologies
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In general capillaries used have an outer coating of polyimide for flexibility. This coating is not UV transparent. For good measurements a small window for detection is made on the capillary most often by burning the coating and cleaning with alcohol.

For reproducible measurements it is necessary that the light beam is always focused on the centre of the capillary. Therefore the CE cell has been designed with a slit capturing the capillary at the window. With this capturing a ball lens is positioned to have the optimum light intensity inside the capillary giving the best signal results. Because of this design a reproducible installation from capillary to capillary is easily achieved. Actually by checking the light intensity going through the capillary itself it is possible to position the window of the capillary at the light beam and optimise for small deviations in the window performance.


Wavelength switching
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For analyses of compounds which differ in their l max. and/or in concentration and therefore in the absorbance at the peak maximum it is useful to switch the detection wavelength and the range during the run according to the migration time of the compound. For complex mixtures the Lambda 1010 offers switching of wavelength.


Detection at the absorbance maximum
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To detect unknown compounds at their maximum absorbance it is recommended to use the l max. wavelength. The Lambda 1010 offers the possibility to measure the UV-spectra of peaks during the run without stop-flow. Measuring first the absorbance of the background electrolyte and then the spectra of the separated compounds you will obtain the peak spectra by a subtraction process.


On column measurement
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Using capillary columns there is the need to detect directly on-column to avoid peak broadening and any other extra column effect. This is possible with the capillary CE cell of the Lambda 1010 where the fused silica separation capillary is mounted in the light beam. The capillary CE cell for the Lambda 1010 is retrofitable. An additional calibration of the detector to adapt the CE cell is not needed. The self aligning optics of the capillary flow cell guarantee the adjustment of different capillary diameters to the optics of the detector.

Due to the small illuminated volume the low noise of ±2x10-5 AU and the high separation efficiency of CZE the mass-sensitivity for Uracil is approx. 1 pg. The capillary acts like a cylindrical lens and gives extraordinarily good linearity for the detector with absorbance < 0.1 AUFS.

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