The 428 Scanner is an epifluorescence confocal microscope that uses a green and red laser to excite fluorophores at 532 and 635 nm respectively. During scanning, two photomultiplier tubes equipped with different filters collect and convert the fluorescent emissions into an electrical current. The 428 Scanner converts this current into a numeric value (0 to 65,535) through an analog to digital converter. These digital intensity values are collected from discrete areas called pixels (10x10 microns) on the array surface and are saved to an image data file (*.tif) ont the computer workstation.
The instrument scans are 10 micron (µm) resolution and also offers a preview scan option (20 or 50 µm resolution). The scan time depends on the number of times each line is scanned (line average setting), the size of the scan area, and the resolution selected.
The Jaguar software enables you to specify up to ten scans per experiment. Each scan may specify a different dye, gain setting, or scan area and generates a separate scan image (16-bit TIFF file, *.tif). The software selects the appropirate laser and filter for the specified dye. If there are multiple scans, the data are saved as sequential numbered images to one image data file.
Shipping110 lbs (50 kg)
Free standing90 lbs (40 kg)
Height~ 15 inches (38.1 ± 0,5 cm)
Width~ 15 inches (38.1 ± 0,5 cm)
Length~ 24 inches (61 cm)
Wattage250 Watts max.
Line Frequency50-60 Hz
Temperature68-85° F (20-30°C)
Altitude2000 meters maximum
PollutionPollution degree 2
Installation categroyInstallation categroy II
Clearance2 in. (5 cm) per side, back and top
American1 x 3 inches (25.4 x 76.2 ± 0,1mm)
Japanese26 x 75 x 1 mm ± 0.1 mm
Metric26 x 75 x 1 mm ± 0.1 mm
Full field scan time50 scan lines/sec. 2.5 min @ 10 µm resolution
Scannable area/slide22 x 75 mm (single-pass/color)